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Invitrogen
Caspase 11 Monoclonal Antibody (17D9), eBioscience™
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图: 1 / 5
Caspase 11 Antibody (14-9935-82) in WB
Lysates prepared under reducing conditions from J774 cells (lane 1) or LPS stimulated mouse splenocytes (lane 2) or unstimulated splenocytes (lanes 3) were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Mouse Caspase 11 Purified.Bands were visualized using Anti-Rat IgG HRP.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
14-9935-82
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
17D9
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody 17D9 recognizes mouse Caspase-11, a cysteine protease that plays a role in apoptosis and inflammation. Caspase-11 exists as a 43 kDa and 38 kDa pro-caspase form and a 30 and 10 kDa active form. The 17D9 antibody recognizes p43, p38 and p30 forms. Caspase-11 is an upstream protease that activates other caspases, such as caspase-3, -7 and -1, which in turn activate IL-1beta. Under Caspase-11-deficient conditions, cells are unable to process or secrete IL-1beta in response to stimuli and show defects in motility, which are due to disruption of its interaction with Aip1, an actin binding protein. Caspase-11 is homologous to human Caspase-4 (around 60%). Unlike other caspases which tend to be constitutively expressed, caspase-11 is upregulated by LPS treatment in macrophages, lymphocytes and hepatocytes.
Applications Reported: This 17D9 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 17D9 antibody has been tested by western blotting at 1-5 µg/mL on J774 cells or stimulated splenocyte lysates. Samples must be reduced for detection. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: caspase 11, apoptosis-related cysteine peptidase; caspase 11, apoptosis-related cysteine protease; caspase 4, apoptosis-related cysteine protease; caspase-11 short form splicing; protease ICH-3; unnamed protein product
基因别名: CASP-11; CASP-4; Casp11; Caspl; ich-3
UniProt ID: (Mouse) P70343
Entrez Gene ID: (Mouse) 12363
proteolysis
apoptotic process
response to stress
actin filament organization
programmed cell death
protein autoprocessing
detection of lipopolysaccharide
positive regulation of interleukin-1 beta production
ectopic germ cell programmed cell death
defense response to bacterium
regulation of apoptotic process
positive regulation of neuron apoptotic process
regulation of inflammatory response
positive regulation of inflammatory response
defense response to Gram-positive bacterium
protein maturation
pyroptosis
pyroptotic cell death
non-canonical inflammasome complex assembly
positive regulation of NLRP3 inflammasome complex assembly
positive regulation of interleukin-18-mediated signaling pathway
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