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Invitrogen
Cathepsin B Polyclonal Antibody
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Cathepsin B Antibody (PA5-47975) in IHC (F)
Immunohistochemical analysis of Cathepsin B in perfusion fixed frozen sections of mouse thymus. Samples were incubated in Cathepsin B polyclonal antibody (Product # PA5-47975) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-47975
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B His18-Phe339
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 25% cross-reactivity with recombinant human Cathepsin B is observed and less than 5% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin C, and rmCathepsin D is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Cathepsin B is a gene located on chromosome 8p23.1 that encodes a lysosomal cysteine protease involved in intracellular protein degradation. As a member of the cathepsin family, Cathepsin B plays a crucial role in the lysosomal pathway, facilitating the breakdown of proteins into amino acids, which can then be recycled for cellular functions. In addition to its proteolytic activity within lysosomes, Cathepsin B is also implicated in extracellular matrix remodeling, cellular invasion, and apoptotic pathways. Its expression and activity are often upregulated in various cancer types, including breast, colon, and pancreatic cancers, where it contributes to tumor progression and metastasis by degrading extracellular matrix components and promoting tumor cell invasion. Due to these roles, Cathepsin B is considered a potential biomarker for cancer prognosis and a target for therapeutic intervention aimed at inhibiting cancer cell dissemination. Research continues to explore its broader implications in diseases related to protease activity and inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cathepsin B; Cathepsin B1
Gene Aliases: CB; Ctsb
UniProt ID: (Mouse) P10605
Entrez Gene ID: (Mouse) 13030
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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