Invitrogen

Collagen X Polyclonal Antibody

3 篇参考文献

查看其他10个Collagen X抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (3)

Collagen X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Collagen X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 3

Collagen X Antibody (PA5-115039) in IHC (P)

Immunohistochemistry analysis of Collagen X in human colorectal cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115039) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Collagen X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Collagen X Antibody in Western Blot (WB)

Collagen X Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Collagen X Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Collagen X in human colorectal cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115039) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Collagen X Antibody in Western Blot (WB) — Western blot analysis of Collagen X in extracts from various samples. Samples were incubated with Collagen X polyclonal antibody (Product # PA5-115039). Lane 1: Hepg2 cells (serum starvation treatment), blocked with antigen-specific peptides; Lane 2: Hepg2 cells (serum starvation treatment); Lane 3: Hela cells (LPS 4h treatment); Lane 4: K562 cells (serum starvation treatment).

Collagen X Antibody in Western Blot (WB) — Western blot analysis of Collagen X in K562 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115039).

Immunogen image

Collagen X Polyclonal Antibody

Immunogen Image Collagen X Polyclonal Antibody

产品信息

PA5-115039

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

-

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human COL10A1(Accession Q03692), corresponding to amino acid residues A636-M680.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total Collagen X.

靶标信息

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Collagen alpha-1(X) chain; pro alpha 1; procollagen, type X, alpha 1

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基因别名: Col10; Col10a-1; COL10A1

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UniProt ID: (Mouse) Q05306

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Entrez Gene ID: (Mouse) 12813, (Rat) 25681

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molecular_function extracellular matrix structural constituent extracellular matrix structural protein

endochondral ossification cartilage development