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Agrisera
CpxA/Conjugative plasmid expression Polyclonal Antibody
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CpxA/Conjugative plasmid expression Antibody (AS13-2653) in WB
Aliquots of 20 µL total cell lysate, derived from 1 mL of pelleted Y. pseudotuberculosis YPIII bacteria (absorbance at 600 nm of ~1.5) that was extracted with 200 l 1 x loading buffer, were separated by 12% acrylamide SDS-PAGE and then wet-blotted for 1 h to Immobilon® -P PVDF membrane (Millipore). Following transfer, the membrane was blocked immediately in milk solution (10% milk powder in TBST) for 1 h a... View More
产品信息
AS13-2653
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Yersinia pseudotuberculosis
靶标信息
CpxA is a bacterial inner-membrane, envelope-stress sensor histidine kinase that spans the cytoplasmic membrane with two transmembrane helices flanking an extracytoplasmic/periplasmic sensor region and a cytosolic transmitter module; the periplasmic sensor adopts a PAS-like (Per-ARNT-Sim) fold and dimerizes as part of signaling control, while the cytoplasmic portion contains the canonical histidine-kinase core (including HAMP, dimerization/phosphotransfer and catalytic/ATP-binding subdomains) that supports autophosphorylation and phosphotransfer. Functionally, CpxA forms a two-component system with the response regulator CpxR, relaying envelope perturbations such as misfolded extracytoplasmic proteins by modulating CpxR phosphorylation state to reprogram transcription of cell-envelope quality-control and biogenesis factors; in Escherichia coli, Cpx pathway activation increases expression of periplasmic folding and degradation machinery including DsbA (disulfide bond formation), DegP (periplasmic protease/chaperone), and peptidyl-prolyl isomerases, thereby maintaining envelope proteostasis and promoting adaptation to extracytoplasmic stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ecf; ecfB; ECK3904; eup; rssE; ssd
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