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CrPDAT1/Phospholipid: diacylglycerol acyltransferase Polyclonal Antibody
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CrPDAT1/Phospholipid: diacylglycerol acyltransferase Antibody (AS12-1875) in WB
Total proteins (containing 2.5 to 30 µg ) from Chlamydomonas cells extracted with lysis buffer (50 m M Tris-HCl, p H 6.8, containing 2% SDS and 10 m M EDTA and a protease inhibitor cocktail) were separated on 10% SDS-PAGE and transferred onto a nitrocellose blot over night at 4°C. Blots were blocked with blocking buffer ( 5% (w/v) non-fat dry milk powder in TBS-T) for 2 hrs at room temperature (RT) with agi... View More
产品信息
AS12-1875
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant CrPDAT1 without transmembrane domains, overexpressed in E.coli.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii
靶标信息
CrPDAT1 encodes a phospholipid:diacylglycerol acyltransferase of the LCAT-like family in the unicellular green alga Chlamydomonas reinhardtii; the protein is synthesized as a soluble, N-terminally targeted enzyme proposed to act at the chloroplast based on the presence of a transit peptide, and recombinant CrPDAT retains activity when its N-terminus is truncated, consistent with a modular organization containing a catalytic core typical of LCAT-like acyltransferases. Functionally, CrPDAT1 catalyzes acyl-CoA-independent triacylglycerol (TAG) formation by transferring an acyl group from membrane phospholipids and galactolipids to diacylglycerol, and it can also mediate DAG:DAG transacylation, thereby diverting fatty acid flux from membrane lipids into storage TAG. In addition to acyltransferase activity, CrPDAT1 exhibits broad lipase/acyl-hydrolase activities toward TAG and multiple membrane lipids, supporting a role in coordinated membrane lipid turnover and remodeling. Genetic perturbation reduces TAG accumulation during nitrogen starvation and alters membrane lipid composition with impaired growth, indicating CrPDAT1 is important both for robust proliferation in favorable conditions and for stress survival by coupling membrane lipid degradation to TAG production.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cr-PDAT; CrPDAT; PDAT; PDAT1; phospholipid:diacylglycerol acyltransferase; phospholipid:diacylglycerol acyltransferase 1
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