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Invitrogen
Cre recombinase Polyclonal Antibody
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Cre recombinase Antibody (PA5-32244) in WB
Cre recombinase Polyclonal Antibody detects Cre recombinase protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72 kDa).7.5 % SDS-PAGE. Cre recombinase Polyclonal Antibody (Product # PA5-32244) dilution: 1:10,000.
产品信息
PA5-32244
应用
建议稀释比
已发表文章
产品规格
种属反应
Virus
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.89 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T transfected recombinant cre protein, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
