Invitrogen

CtBP2 Polyclonal Antibody

Name: CtBP2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79086

高级验证

查看其他23个CtBP2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (17)
高级验证 (1)

CtBP2 Antibody in Immunocytochemistry (ICC/IF)

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CtBP2 Antibody (PA5-79086) in ICC/IF

Immunocytochemistry analysis of CTBP2 using anti-CTBP2 antibody (Product # PA5-79086) . CTBP2 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CTBP2 antibody (Product # PA5-79086) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. V... View More

CtBP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CtBP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CtBP2 was observed in OVCAR-3 and MOLT-4 in comparison to Raji using Anti-CtBP2 polyclonal antibody (Product # PA5-79086) in Western Blot. {RE}

产品信息

PA5-79086

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human CTBP2 recombinant protein (Position: H321-Q445).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746202

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human A549 whole cell, human PC-3 whole cell, human THP-1 whole cell, human HEK293 whole cell, human CACO-2 whole cell, human HELA whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: Human Intestinal Cancer tissue, Rat Skeletal Muscle tissue, Mouse Skeletal Muscle tissue. ICC/IF: U20S cell, U20S cell. Flow: SiHa cell, A431 cell.

靶标信息

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21. 3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10.

仅用于科研。不用于诊断过程。未经明确授权不得转售。