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Rockland
Cyanine Polyclonal Antibody, AP
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Cyanine Antibody (600-605-209) in DB
Dot Blot of Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Cy3 Streptavidin Conjugated Row 2 - Cy5 Streptavidin Conjugated. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.
产品信息
600-605-209
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A mixture of Cy3 and Cy5 conjugated KLH
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.05M tris HCl, pH 8, with 10mg/mL BSA, 0.15M NaCl, 0.0001M ZnCl2, 0.001M MgCl2, 50% glycerol
内含物
0.01% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
靶标信息
Cyanines are blue-green fluorescent dyes belonging to polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5 and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for 2 color detection in Flow Cytometry applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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