Rockland

Cyclin L2 Polyclonal Antibody

Name: Cyclin L2 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-878

查看其他5个Cyclin L2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

Cyclin L2 Antibody (600-401-878) in WB

Western blot using Rocklands Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ... View More

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Immunogen image

Immunogen Image Cyclin L2 Polyclonal Antibody

产品信息

600-401-878

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

酶联免疫吸附实验 (ELISA)

1:2,000-1:10,000

免疫沉淀 (IP)

1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80% homology with the protein from these sources. Cross reactivity with Cyclin L2a homologues from other sources has not been determined.

靶标信息

CCNL2 belongs to the cyclin family, Cyclin L subfamily. CCNL2 is a transcriptional regulator which participates in regulating the pre-mRNA splicing process. CCNL2 also modulates the expression of critical apoptotic factor, leading to cell apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cyclin Ania-6b; cyclin M; cyclin S; Cyclin-L2; paneth cell enhanced expression; Paneth cell-enhanced expression protein; PCEE

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基因别名: 1700010A01Rik; 1810019L15Rik; 2010319M22Rik; AA409936; ANIA-6B; Ania6b; AW261738; CCNL2; CCNM; CCNS; HCLA-150; HCLA-ISO; HLA-ISO; MNCb-5160; PCEE; SB138

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UniProt ID: (Human) Q96S94, (Mouse) Q9JJA7

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Entrez Gene ID: (Human) 81669, (Mouse) 56036

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功能

protein binding cyclin-dependent protein serine/threonine kinase regulator activity protein kinase binding kinase activator

参与通路

transcription, DNA-templated positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription from RNA polymerase II promoter positive regulation of phosphorylation of RNA polymerase II C-terminal domain regulation of cyclin-dependent protein serine/threonine kinase activity regulation of transcription, DNA-templated biological_process