Rockland

Cyclin T2 Polyclonal Antibody

Name: Cyclin T2 Polyclonal Antibody
Brand: Rockland
SKU: 100-401-931

查看其他4个Cyclin T2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

Cyclin T2 Antibody (100-401-931) in WB

Western blot using Rocklands Anti-Cyclin T2 (Rabbit) is shown to detect two major bands (arrowheads) corresponding to human Cyclin T2a (~74kDa) and T2b (~81kDa) as indicated. Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% BLOTTO (p/n B501-0500) in PBS, the membrane was probed for overnight at 4° C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG... View More

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Immunogen image

Immunogen Image Cyclin T2 Polyclonal Antibody

产品信息

100-401-931

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:5,000-1:20,000

免疫沉淀 (IP)

1:500-1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-584 of human Cyclin T2a protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

85 mg/mL

保存液

whole serum

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is directed against human Cyclin T2 proteins. Antiserum will specifically react with proteins 74 and 81 kDa in size corresponding to isoforms of Cyclin T2 protein by western blot. A protein:protein BLAST analysis was used to suggest cross reactivity with Cyclin T2a and T2b from dog (96%), bovine (93%), mouse (88%) and rat (85%) sources based on high homology with the full length protein. Reactivity against homologues from other sources is not known.

靶标信息

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. A pseudogene of this gene is found on chromosome 1. Alternate splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: cyclin T2a; cyclin T2b; Cyclin-T2; CycT2; SDS-stable vimentin-bound DNA fragment HEF42VIM22; subunit of positive elongation transcription factor b

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基因别名: CCNT2; CYCT2

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UniProt ID: (Human) O60583

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Entrez Gene ID: (Human) 905

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功能

transcription coactivator binding chromatin binding protein serine/threonine kinase activity protein binding cyclin-dependent protein serine/threonine kinase regulator activity RNA polymerase binding 7SK snRNA binding kinase activator

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