Invitrogen

Cyclophilin B Monoclonal Antibody (11C11)

Name: Cyclophilin B Monoclonal Antibody (11C11)
Brand: Invitrogen
SKU: MA5-49248

查看其他17个Cyclophilin B抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (9)

Cyclophilin B Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

Cyclophilin B Antibody (MA5-49248) in ICC/IF

Immunocytochemistry analysis of Cyclophilin B in U2OS cells. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The cells were blocked with 10% goat serum. Samples were then incubated in Cyclophilin B Monoclonal antibody (Product # MA5-49248) using a dilution of 2 μg/mL. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 m... View More

Cyclophilin B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclophilin B Antibody in Western Blot (WB)

Cyclophilin B Antibody in Flow Cytometry (Flow)

Product Image Gallery

Immunogen image

Immunogen Image Cyclophilin B Monoclonal Antibody (11C11)

产品信息

MA5-49248

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

11C11

抗原

E. coli-derived human Cyclophilin B recombinant protein (Position: K158-E216).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074906

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human placenta tissue, U-87MG whole cell, HepG2 whole cell, Caco-2 whole cell, SW620 whole cell, PANC-1 whole cell, THP-1 whole cell, HEK293 whole cell, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue. IHC: human oesophagus squama cancer tissue, human ovarian cancer tissue, human placenta tissue, human tonsil tissue, human lung cancer tissue. ICC/IF: U20S cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Cyclophilin B; cyclophilin-like protein; Cyp B; CYP-S1; EC 5.2.1.8; epididymis secretory protein Li 39; Peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase; PPIase B; Rotamase; Rotamase B; S-cyclophilin; S-cyclophylin; SCYLP

查看更多收起

基因别名: AA408962; AA553318; AI844835; Cphn-2; Cphn2; CyP-20b; CYP-S1; CYPB; HEL-S-39; OI9; PPIB; SCYLP

查看更多收起

UniProt ID: (Human) P23284, (Rat) P24368, (Mouse) P24369

查看更多收起

Entrez Gene ID: (Human) 5479, (Rat) 64367, (Mouse) 19035

查看更多收起

功能