Search
Invitrogen
DDX1 Monoclonal Antibody (3I10)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 13
DDX1 Antibody (MA5-49262) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of DDX1 in Hela cells using DDX1 Monoclonal Antibody (3I10) (Product # MA5-49262). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualized using a fluorescence microscope and filter sets appropriate for the label used.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-49262
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3I10
抗原
E.coli-derived human DDX1 recombinant protein (Position: K562-F740).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human CACO-2 whole cell, human U20S whole cell, human HEK293 whole cell, human U87 whole cell, human HELA whole cell, human A549 whole cell, rat heart tissue, rat kidney tissue, rat skeletal muscle tissue, mouse kidney tissue. IHC: human prostatic acinar adenocarcinoma tissue, human colorectal adenocarcinoma tissue, human ovarian cancer tissue, human spleen tissue, human squamous cell cervical carcinoma tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, rat brain tissue. ICC/IF: Hela cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
基因别名: AA409185; DBP-RB; DDX1
UniProt ID: (Rat) Q641Y8, (Mouse) Q91VR5
Entrez Gene ID: (Rat) 84474, (Mouse) 104721
DNA binding
chromatin binding
transcription cofactor activity
double-stranded RNA binding
nuclease activity
exonuclease activity
ATP binding
ATP-dependent helicase activity
poly(A) binding
DNA/RNA helicase activity
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
ATP-dependent RNA helicase activity
helicase activity
protein binding
hydrolase activity
double-strand break repair
transcription, DNA-templated
regulation of transcription, DNA-templated
tRNA splicing, via endonucleolytic cleavage and ligation
mRNA processing
multicellular organism development
response to virus
DNA duplex unwinding
response to exogenous dsRNA
nucleic acid phosphodiester bond hydrolysis
tRNA processing
RNA secondary structure unwinding
regulation of nucleic acid-templated transcription
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
