Invitrogen

DNA2 Polyclonal Antibody

查看其他7个DNA2抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (3)

DNA2 Antibody in Immunocytochemistry (ICC/IF)

DNA2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

DNA2 Antibody (PA5-115131) in ICC/IF

Immunocytochemistry analysis of DNA2 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115131). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

DNA2 Antibody in Immunocytochemistry (ICC/IF)

DNA2 Antibody in Immunocytochemistry (ICC/IF)

DNA2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

DNA2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DNA2 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115131). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

DNA2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DNA2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115131). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

DNA2 Antibody in Western Blot (WB) — Western blot analysis of DNA2 in Colo205 (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115131).

Immunogen image

DNA2 Polyclonal Antibody

Immunogen Image DNA2 Polyclonal Antibody

产品信息

PA5-115131

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human DNA2(Accession P51530), corresponding to amino acid residues T430-G480.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total DNA2L.

靶标信息

DNA2 is a conserved helicase/nuclease involved in themaintenance of mitochondrial and nuclear DNA stability.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DNA replication ATP-dependent helicase-like homolog; DNA replication ATP-dependent helicase/nuclease DNA2; DNA2-like helicase; hDNA2

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基因别名: DNA2; DNA2L; E130315B21Rik; KIAA0083

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UniProt ID: (Mouse) Q6ZQJ5

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Entrez Gene ID: (Mouse) 327762

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nucleotide binding DNA binding catalytic activity helicase activity nuclease activity endonuclease activity protein binding ATP binding hydrolase activity site-specific endodeoxyribonuclease activity, specific for altered base 5'-flap endonuclease activity phosphoric ester hydrolase activity 5'-3' DNA helicase activity single-stranded DNA-dependent ATPase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity metal ion binding iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding

DNA replication checkpoint DNA double-strand break processing DNA replication mitochondrial DNA replication DNA repair base-excision repair cellular response to DNA damage stimulus metabolic process DNA duplex unwinding DNA replication, Okazaki fragment processing DNA replication, removal of RNA primer mitochondrial DNA repair positive regulation of DNA replication nucleic acid phosphodiester bond hydrolysis