Invitrogen

DNM1L Polyclonal Antibody

Name: DNM1L Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-16987

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12个已发表图片

13 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

DNM1L Antibody in Immunocytochemistry (ICC/IF)

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DNM1L Antibody (PA1-16987) in ICC/IF

Immunocytochemistry analysis of DNM1L in HeLa cells. Samples were incubated with DNM1L polyclonal antibody (Product # PA1-16987) using a dilution of 5.0 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Alpha tubulin (DM1A) was used as a co-stain at 1:1,000 and detected with an anti-mouse Dylight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged u... View More

DNM1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNM1L Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with DNM1L siRNA and reduction of signal was observed in Western Blot using DNM1L Polyclonal Antibody (Product # PA1-16987). {KD}

产品信息

PA1-16987

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

2.5 µg/mL

免疫细胞化学 (ICC/IF)

1:500

流式细胞分析 (Flow)

1:1,000

其他PubMed (Misc)

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产品规格

种属反应

Fish, Human, Mouse, Non-human primate, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

AB_2093523

产品详细信息

This antibody is predicted to react with bovine based on 100% sequence homology.

Suggested positive control: antigen standard for DNM1L (transient overexpression lysate), human kidney protein.

靶标信息

A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; N-terminal region; VPS1

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基因别名: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; HDYNIV; python

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UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6

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Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006

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功能