Invitrogen

DRD1 Recombinant Rabbit Monoclonal Antibody (1H8L2)

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问题与答案

问题与答案

抗体检测数据 (4)
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DRD1 Antibody in Immunocytochemistry (ICC/IF)

DRD1 Antibody in Immunocytochemistry (ICC/IF)

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DRD1 Antibody (702593) in ICC/IF

For immunocytochemistry analysis, HEK 293 cells stably transfected with Dopamine D1 receptor (D1R) were grown on poly-L-lysine-coated coverslips overnight. Cells were then treated with 10 µM dopamine for 30 min, washed with PBS and fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti- DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL)... View More

DRD1 Antibody in Immunocytochemistry (ICC/IF)

DRD1 Antibody in Immunocytochemistry (ICC/IF)

DRD1 Antibody in Immunohistochemistry (IHC)

DRD1 Antibody in Western Blot (WB)

DRD1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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DRD1 Antibody in Immunocytochemistry (ICC/IF) — For immunocytochemistry analysis, HEK 293 cells stably transfected with Dopamine D1 receptor (D1R) were grown on poly-L-lysine-coated coverslips overnight. Cells were then treated with 10 µM dopamine for 30 min, washed with PBS and fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti- DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL). Cells were then incubated with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, robust receptor internalization after agonist exposure.

DRD1 Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, HEK-293 cells were stably transfected with human DRD1. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous DRD1 using Anti- DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of the DRD1 on the transfected cells. The images were captured at 40X magnification.

DRD1 Antibody in Immunohistochemistry (IHC) — Sections of Rat brain striatum & hypothalamus were dewaxed, microwaved in citric acid and incubated with Anti-DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a & b) shows the specific localization of DRD1 in neuronal stomata and dendrites in striatum and hypothalamus respectively. Panel c) shows the no primary antibody control.

DRD1 Antibody in Western Blot (WB) — Western blot analysis was performed on HEK-293 cells stably transfected with human DRD1 (Lane 2) and Mock (Lane 1). Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. The blot was probed with Anti-DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL, 1:500 dilution). An ~70-100 kDa band corresponding to DRD1 was observed in the transfected cell line alone.

DRD1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 4. Trans -dual nicks with RecOFAR facilitates efficient gene KI in rats. ( A ) Schematic overview of the strategy to generate a GFAP-p2A-mEYFP KI allele. The nested PCR primers used for knock-in identification are shown ( Supplementary Table S4 ). Two targets are shown with green. The restriction endonuclease used for Southern blotting is SphI. A 3' internal probe on 3' homology arm is shown. ( B ) Germline transmission rate of p2A-mEYFP precise integration at the gfap locus without and with RecOFAR. ( C ) Immunostaining brain sections of gfap KI-positive F 1 rats. Neurons marked with NeuN are in red, mEYFP is in green and glial cells marked with anti-GFAP are in white. Scale bar in left panel is 200 mum. The region in the box is magnified in the right panels. Scale bar, 100 mum. ( D ) Southern blotting analysis of the gfap targeted allele and WT. T, KI target band, 6.6 kb. ( E ) Schematic overview of the strategy to generate the Drd2-p2A-ChR2-EYFP, Drd1-p2A-ChR2-EYFP and Bassoon -5.5 kb KI alleles. The restriction endonuclease HindIII and KpnI were used for Southern blotting at the Drd2 and Drd1 loci respectively. Two 3' internal probes on 3' homology arms are shown at the Drd2 and Drd1 loci. The PCR primers used for knock-in identification are shown ( Supplementary Table S4 ). ( F ) Germline transmission rate of p2A-ChR2-EYFP precise integration at the Drd2 locus with different strategies. ( G ) Southern blotting analysis of the Drd2-p2A-ChR2-EYFP targeted allele. (

Immunogen image

DRD1 Recombinant Rabbit Monoclonal Antibody (1H8L2)

Immunogen Image DRD1 Recombinant Rabbit Monoclonal Antibody (1H8L2)

产品信息

702593

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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免疫组化 (IHC)

1:500

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免疫细胞化学 (ICC/IF)

1:500

-

产品规格

种属反应

Human, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1H8L2

抗原

Peptide corresponding to human Dopamine Receptor D1 [aa427-aa446]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

This antibody is predicted to react with Monkey, Cat, Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: D(1A) dopamine receptor; D1a receptor; Dopamine 1 receptor; Dopamine D1 receptor; dopamine receptor D1A; Dopamine-1A receptor

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基因别名: D1a; DADR; Drd-1; DRD1; DRD1A

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UniProt ID: (Human) P21728

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Entrez Gene ID: (Human) 1812, (Rat) 24316

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dopamine neurotransmitter receptor activity, coupled via Gs G-protein alpha-subunit binding dopamine neurotransmitter receptor activity protein binding drug binding protein phosphatase binding angiotensin receptor binding D3 dopamine receptor binding protein complex binding dopamine binding protein heterodimerization activity ATPase binding G-protein coupled receptor activity receptor binding G-protein coupled receptor

temperature homeostasis conditioned taste aversion behavioral fear response synaptic transmission, dopaminergic response to amphetamine negative regulation of protein kinase activity protein import into nucleus G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-activating G-protein coupled receptor signaling pathway activation of adenylate cyclase activity adenylate cyclase-activating dopamine receptor signaling pathway synapse assembly learning memory mating behavior grooming behavior adult walking behavior visual learning positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway astrocyte development response to activity dopamine transport transmission of nerve impulse neuronal action potential regulation of vasoconstriction calcium-mediated signaling dentate gyrus development striatum development orbitofrontal cortex development cerebral cortex GABAergic interneuron migration positive regulation of cell migration negative regulation of cell migration peristalsis positive regulation of cAMP biosynthetic process response to food response to estradiol response to retinoic acid cellular response to insulin stimulus response to nicotine operant conditioning social behavior response to antidepressant regulation of dopamine metabolic process vasodilation negative regulation of circadian sleep/wake cycle, sleep dopamine metabolic process response to drug maternal behavior response to amino acid positive regulation of potassium ion transport response to morphine response to ethanol positive regulation of membrane potential glucose import habituation sensitization behavioral response to cocaine regulation of long-term neuronal synaptic plasticity response to steroid hormone positive regulation of release of sequestered calcium ion into cytosol positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway regulation of dopamine uptake involved in synaptic transmission positive regulation of synaptic transmission, glutamatergic prepulse inhibition phospholipase C-activating dopamine receptor signaling pathway long-term synaptic potentiation long term synaptic depression cellular response to hypoxia cellular response to catecholamine stimulus positive regulation of long-term synaptic potentiation positive regulation of feeding behavior startle response intracellular protein transport muscle contraction G-protein coupled receptor signaling pathway dopamine receptor signaling pathway chemical synaptic transmission locomotory behavior feeding behavior associative learning response to organonitrogen compound response to organic cyclic compound hippocampus development phosphatidylinositol catabolic process response to cocaine regulation of ion transport positive regulation of adenylate cyclase activity phosphatidylinositol metabolic process