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Invitrogen
EGFR Antibody Cocktail
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EGFR Antibody (MA5-13343) in ICC/IF
Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (111.6+199.12+ F4+11E8) Mouse Monoclonal Antibody (Product # MA5-13343) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
产品信息
MA5-13343
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1/IgG2a
分类
Cocktail
类型
Antibody
克隆号
111.6, 199.12, F4, 11E8
抗原
Extracellular and cytoplasmic domains of recombinant human EGFR protein
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Wet ice
RRID
产品详细信息
MA5-13343 targets Epidermal Growth Factor Receptor in IP and WB applications and shows reactivity with Human samples.
The MA5-13343 immunogen is extracellular and cytoplasmic domains of recombinant human EGFR protein.
This cocktail is also referred to as clone R19/48.
靶标信息
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
