Invitrogen

EGR1 Recombinant Rat Monoclonal Antibody (HEGR1DS), Alexa Fluor™ Plus 647

Name: EGR1 Recombinant Rat Monoclonal Antibody (HEGR1DS), Alexa Fluor\u2122 Plus 647
Brand: Invitrogen
SKU: 740183TP647

高级验证

查看其他27个EGR1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (3)

EGR1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

EGR1 Antibody (740183TP647) in ICC/IF

Immunofluorescence analysis of EGR1 was performed using EGR1 Recombinant Rat Monoclonal Antibody (HEGR1DS) Alexa Fluor™ Plus 647 (Product # 740183TP647) on 70-80% confluent U-87 MG cells treated with PMA (100 ng/mL for 2 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilised with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with EGR1 Recombinant Rat Monoclonal Antibody (HEGR1DS) Alexa Fluor™ Plus 647 (Product # 740183TP647, 1:200) incubated at 4... View More

Product Image Gallery

EGR1 Antibody — Detection of altered expression of the target protein by cell treatment demonstrates antibody specificity. EGR1 Recombinant Rat Monoclonal Antibody (HEGR1DS), Alexa Fluor™ Plus 647 (Product # 740183TP647) specifically shows increased expression of EGR1 in treated cells (pink histogram) as compared to untreated cells (blue histogram). {TM}

产品信息

740183TP647

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

1 µg/test

产品规格

种属反应

Human

宿主/亚型

Rat / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

HEGR1DS

偶联物

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

激发/发射光谱

658/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: AT225; Early growth response protein 1; EGR-1; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24

查看更多收起

基因别名: AT225; EGR1; G0S30; KROX-24; KROX24; NGFI-A; TIS8; ZIF-268; ZNF225

查看更多收起

UniProt ID: (Human) P18146

查看更多收起

Entrez Gene ID: (Human) 1958

查看更多收起

功能