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Invitrogen
EHD3 Polyclonal Antibody
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EHD3 Antibody (PA5-143924) in ICC/IF
Immunocytochemistry analysis of EHD3 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in EHD3 Polyclonal antibody (Product # PA5-143924) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
产品信息
PA5-143924
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human EHD3 (NELPAHLLPPSKRKVAE).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human A549 whole cell, human Hela whole cell, human CCRF-CEM whole cell, human U-87MG whole cell, human SY-SY5Y whole cell, monkey heart tissue, rat heart tissue, rat brain tissue, rat kidney tissue, rat liver tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, mouse liver tissue. IHC: mouse brain tissue, human ovarian serous adenocarcinoma tissue, human ovarian serous adenocarcinoma tissue, human renal clear cell carcinoma tissue, human breast cancer tissue, human lung cancer tissue, human rectal cancer tissue, human gastric adenocarcinoma tissue, rat brain tissue. ICC/IF: MCF-7 cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The EHD3 gene encodes the EH-domain containing protein 3, part of the EHD protein family involved in the regulation of endocytic recycling and membrane trafficking. EHD3 plays a crucial role in the endosomal transport system, impacting how receptors and other membrane proteins are recycled back to the cell surface after internalization. This protein aids in maintaining cellular homeostasis by ensuring the efficient sorting and transport of cargo through the endosomal system. EHD3 has been implicated in various cellular functions, including insulin signaling, receptor recycling, and neuronal development, and significantly influences synaptic transmission and plasticity. Alterations in EHD3 expression or function can disrupt endosomal trafficking processes and are linked to conditions such as neurodegenerative diseases and cancer. Research on EHD3 is focused on uncovering its role in membrane dynamics and identifying potential therapeutic strategies for disorders involving dysfunctional endocytic pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EH domain-containing protein 3; EH-domain containing 3; EH-domain containing protein 2; PAST homolog 3
基因别名: EHD2; EHD3; PAST3
UniProt ID: (Human) Q9NZN3, (Rat) Q8R491, (Mouse) Q9QXY6
Entrez Gene ID: (Human) 30845, (Rat) 192249, (Mouse) 57440
