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ERK1/ERK2 Antibody (710214) in WB
Western blot analysis of ERK1/2 was performed by loading 20 µg of Raji (lane1), HeLa (lane2), Jurkat (lane3), MDA-MB-231 (lane4) and MCF7 (lane5) cell lysates using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. ERK1/2 was detected at ~43 kDa using... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
710214
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
3HCLC
抗原
Peptide corresponding to amino acids 293-315 of human extracellular signal regulated kinase 1/2
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with equine, mouse and rat based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; unnamed protein product
基因别名: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MNK1; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3
UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708
Entrez Gene ID: (Human) 5594, (Human) 5595, (Rat) 116590, (Rat) 50689
phosphotyrosine binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
phosphatase binding
identical protein binding
protein serine kinase activity
DNA-binding transcription factor binding
double-stranded DNA binding
protein kinase binding
mitogen-activated protein kinase kinase kinase binding
scaffold protein binding
MAPK cascade
negative regulation of T cell mediated immune response to tumor cell
protein phosphorylation
cellular response to DNA damage stimulus
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
response to toxic substance
organ morphogenesis
positive regulation of macrophage chemotaxis
neural crest cell development
Schwann cell development
phosphorylation
peptidyl-serine phosphorylation
sensory perception of pain
arachidonic acid metabolic process
regulation of ossification
BMP signaling pathway
thyroid gland development
positive regulation of cyclase activity
lipopolysaccharide-mediated signaling pathway
positive regulation of telomere maintenance
response to lipopolysaccharide
regulation of stress-activated MAPK cascade
cellular response to amino acid starvation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
ERBB2-ERBB3 signaling pathway
outer ear morphogenesis
myelination
signal transduction in response to DNA damage
response to exogenous dsRNA
positive regulation of translation
positive regulation of transcription from RNA polymerase II promoter
decidualization
insulin-like growth factor receptor signaling pathway
thymus development
modulation of synaptic transmission
cartilage development
stress-activated MAPK cascade
regulation of intracellular pH
regulation of cytoskeleton organization
Bergmann glial cell differentiation
face development
lung morphogenesis
trachea formation
cardiac neural crest cell development involved in heart development
interleukin-34-mediated signaling pathway
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
interleukin-1-mediated signaling pathway
response to epidermal growth factor
cellular response to mechanical stimulus
cellular response to tumor necrosis factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
negative regulation of cholesterol efflux
cellular response to toxic substance
positive regulation of macrophage proliferation
positive regulation of neuroinflammatory response
negative regulation of TORC1 signaling
positive regulation of xenophagy
regulation of early endosome to late endosome transport
regulation of transcription from RNA polymerase II promoter
apoptotic process
chemotaxis
signal transduction
chemical synaptic transmission
learning or memory
positive regulation of peptidyl-threonine phosphorylation
response to nicotine
ERBB signaling pathway
positive regulation of skeletal muscle tissue regeneration
positive regulation of cholesterol biosynthetic process
positive regulation of smoothened signaling pathway
chemokine-mediated signaling pathway
heart development
positive regulation of cell proliferation
diadenosine tetraphosphate biosynthetic process
cytosine metabolic process
positive regulation of cell migration
response to estradiol
response to testosterone
mammary gland epithelial cell proliferation
cellular response to platelet-derived growth factor stimulus
response to cocaine
positive regulation of protein import into nucleus
response to hydrogen peroxide
response to estrogen
estrous cycle
negative regulation of cell differentiation
positive regulation of transcription, DNA-templated
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of cardiac muscle cell proliferation
long-term synaptic potentiation
labyrinthine layer blood vessel development
cellular response to methionine
cellular response to cAMP
cellular response to epidermal growth factor stimulus
cellular response to peptide hormone stimulus
cellular response to prostaglandin E stimulus
cellular response to lipid
response to alcohol
cellular response to insulin-like growth factor stimulus
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