Proteintech

FN3K Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

抗体检测数据 (9)

FN3K Antibody in Immunocytochemistry (ICC/IF)

FN3K Antibody in Immunocytochemistry (ICC/IF)

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FN3K Antibody (14293-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

FN3K Antibody in Immunocytochemistry (ICC/IF)

FN3K Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FN3K Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FN3K Antibody in Western Blot (WB)

FN3K Antibody in Western Blot (WB)

FN3K Antibody in Western Blot (WB)

FN3K Antibody in Western Blot (WB)

FN3K Antibody in Western Blot (WB)

FN3K Antibody in Immunoprecipitation (IP)

Product Image Gallery

FN3K Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

FN3K Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

FN3K Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

FN3K Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FN3K Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FN3K Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FN3K Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FN3K Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FN3K Antibody in Immunoprecipitation (IP) — IP result of anti-FN3K (IP:14293-1-AP, 3ug; Detection:14293-1-AP 1:500) with HeLa cells lysate 1840ug.

Immunogen image

FN3K Polyclonal Antibody

Immunogen Image FN3K Polyclonal Antibody

产品信息

14293-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

-

免疫组化（石蜡） (IHC (P))

1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:500

-

免疫沉淀 (IP)

0.5-4.0 µg

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

FN3K Fusion Protein Ag5617 (1-309 aa encoded by BC042680)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.19 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: MEQLLRAEL RTATLRAFGG PGAGCISEGR AYDTDAGPVF VKVNRRTQAR QMFEGEVASL EALRSTGLVR VPRPMKVIDL PGGGAAFVME HLKMKSLSSQ ASKLGEQMAD LHLYNQKLRE KLKEEENTVG RRGEGAEPQY VDKFGFHTVT CCGFIPQVNE WQDDWPTFFA RHRLQAQLDL IEKDYADREA RELWSRLQVK IPDLFCGLEI VPALLHGDLW SGNVAEDDVG PIIYDPASFY GHSEFELAIA LMFGGFPRSF FTAYHRKIPK APGFDQRLLL YQLFNYLNHW NHFGREYRSP SLGTMRRLLK (1-309 aa encoded by BC042680)

靶标信息

FN3K catalyzes phosphorylation of fructosamines formed by glycation, the nonenzymatic reaction of glucose with primary amines followed by Amadori rearrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and result in deglycation of glycated proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Fructosamine-3-kinase; Protein-psicosamine 3-kinase FN3K; Protein-ribulosamine 3-kinase FN3K

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基因别名: 2310074G21Rik; FN3K

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UniProt ID: (Human) Q9H479, (Mouse) Q9ER35

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Entrez Gene ID: (Human) 64122, (Mouse) 63828

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kinase activity fructosamine-3-kinase activity phosphofructokinase activity phosphorylase kinase regulator activity cobinamide kinase activity phytol kinase activity phosphatidylinositol phosphate kinase activity cyclin-dependent protein serine/threonine kinase regulator activity transferase activity transferase activity, transferring phosphorus-containing groups phenol kinase activity cyclin-dependent protein kinase activating kinase regulator activity inositol tetrakisphosphate 2-kinase activity heptose 7-phosphate kinase activity aminoglycoside phosphotransferase activity phosphatidylinositol 3-kinase activity eukaryotic elongation factor-2 kinase regulator activity eukaryotic elongation factor-2 kinase activator activity LPPG:FO 2-phospho-L-lactate transferase activity cytidine kinase activity glycerate 2-kinase activity (S)-lactate 2-kinase activity phosphoserine:homoserine phosphotransferase activity L-seryl-tRNA(Sec) kinase activity phosphocholine transferase activity polynucleotide 5'-hydroxyl-kinase activity ATP-dependent polynucleotide kinase activity GTP-dependent polynucleotide kinase activity farnesol kinase activity CTP:2-trans,-6-trans-farnesol kinase activity geraniol kinase activity geranylgeraniol kinase activity CTP:geranylgeraniol kinase activity prenol kinase activity 1-phosphatidylinositol-5-kinase activity 1-phosphatidylinositol-3-phosphate 4-kinase activity phosphatidylinositol-3,4-bisphosphate 5-kinase activity phosphatidylinositol bisphosphate kinase activity inositol-3,4,6-trisphosphate 1-kinase activity inositol 5-diphosphate pentakisphosphate 5-kinase activity inositol diphosphate tetrakisphosphate kinase activity kinase

phosphorylation fructosamine metabolic process fructoselysine metabolic process epithelial cell differentiation post-translational protein modification