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Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Species Reactivity: The antiserum does not cross-react with any other Human plasma proteins as tested in gel diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 µL human plasma or equivalent against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion (Laurell) use a concentration of 1% of the antiserum in the gel.
Factor H is a serum protein acting as an inhibitor in the complement activation system. I activates the C3/C5 convertase by dissociating its subunits and also acts as a cofactor for the enzyme activity of Factor I.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: adrenomedullin binding protein; age-related maculopathy susceptibility 1; beta-1-H-globulin; beta-1H; Complement factor H; complement factor H, isoform b; factor H; factor H-like 1; FHL1 antibody; H factor 1; H factor 1 (complement); H factor 2 (complement); HGNC:4883; MGC88246; RP1-177P10.1
基因别名: AHUS1; AMBP1; ARMD4; ARMS1; CFH; CFHL3; FH; FHL1; HF; HF1; HF2; HUS
UniProt ID: (Human) P08603
Entrez Gene ID: (Human) 3075