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Invitrogen
GFAP Recombinant Mouse Monoclonal Antibody (GA5), Alexa Fluor™ Plus 488
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GFAP Antibody (750024MP488) in ICC/IF
Immunofluorescent analysis of GFAP was performed using GFAP Recombinant Mouse Monoclonal Antibody (GA5), Alexa Fluor™ Plus 488 (Product # 750024MP488) in 70-80% confluent U-251 MG and HeLa cells lines. The cell lines were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GFAP Recombinant Mouse Monoclonal Antibody (GA5), Alexa Fluor™ Plus 488 (Product # 750024MP488, 1:200) in 0.1% BSA, incubated at 4 degree Ce... View More
产品信息
750024MP488
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
GA5
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP)
基因别名: ALXDRD; GFAP
UniProt ID: (Human) P14136
Entrez Gene ID: (Human) 2670
response to wounding
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
regulation of protein complex assembly
intermediate filament organization
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
long-term synaptic potentiation
regulation of chaperone-mediated autophagy
