Invitrogen

GLIS3 Polyclonal Antibody

Name: GLIS3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-41677

高级验证

2个已发表图片

2 篇参考文献

查看其他5个GLIS3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

GLIS3 Antibody in Immunohistochemistry (IHC)

图: 1 / 5

GLIS3 Antibody (PA5-41677) in IHC

Immunohistochemistry analysis of human pancreas cells using an anti-GLIS3 polyclonal antibody (Product # PA5-41677). Primary Antibody Dilution:1:10; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1:1000.

Product Image Gallery

GLIS3 Antibody — Figure 3 miR-106a-5p Inhibits GLIS3 Expression by Targeting its 3'-UTR (A and B) Immunoblot analysis of chondrocytes transfected with miR-106a-5p (A) or anti-miR-106a-5p (B). The histograms shown under the immunoblot graphs reveal corresponding miR-106a-5p expression levels. (C) miRNA luciferase reporter assay in human chondrocytes cotransfected with wild-type or mutated GLIS3 reporter and miR-106a-5p. The top panel shows the wild-type and mutant forms of the putative miR-106a-5p target sequences in the GLIS3 3'-UTR. Bold and red italicized font indicate the putative miR-106a-5p binding sites within the human GLIS3 3'-UTR. GLIS3 WT, wild-type GLIS3 3'-UTR; GLIS3 Mut, mutated GLIS3 3'UTR. Each bar represents the mean +- SD of at least three independent experiments performed in triplicate. *p < 0.05, **p < 0.01. {KD}

Immunogen image

Immunogen Image GLIS3 Polyclonal Antibody

产品信息

PA5-41677

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

1:10

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the N-terminal of human GLIS3 (aa 11-60).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2606554

产品详细信息

Peptide sequence: PPASQVSTAC NQISPSLQRA MNAANLNIPP SDTRSLISRE SLASTTLSLT

Sequence homology: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%

靶标信息

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。