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Invitrogen
GRB2 Polyclonal Antibody
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FIGURE: 1 / 7
GRB2 Antibody (PA5-17692) in ICC/IF
Immunofluorescence analysis of GRB2 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRB2 Rabbit Polyclonal Antibody(Product # PA5-17692) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Product Details
PA5-17692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to carboxy-terminal residues of human GRB2
Conjugate
Form
Liquid
Concentration
217 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2
Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2
UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994
Entrez Gene ID: (Human) 2885, (Bovine) 535298, (Chinese hamster) 100769627, (Mouse) 14784, (Rat) 81504
SH3/SH2 adaptor activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH3 domain binding
protein kinase binding
protein phosphatase binding
identical protein binding
insulin receptor substrate binding
poly(A) RNA binding
ephrin receptor binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
phosphotyrosine binding
protein domain specific binding
phosphoprotein binding
scaffold/adaptor protein
MAPK cascade
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
cell-cell signaling
axon guidance
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
positive regulation of signal transduction
regulation of phosphatidylinositol 3-kinase signaling
viral process
positive regulation of actin filament polymerization
T cell costimulation
receptor internalization
phosphatidylinositol-3-phosphate biosynthetic process
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB2 signaling pathway
negative regulation of epidermal growth factor receptor signaling pathway
signal transduction in response to DNA damage
regulation of MAPK cascade
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
anatomical structure formation involved in morphogenesis
leukocyte migration
branching involved in labyrinthine layer morphogenesis
cellular response to ionizing radiation
positive regulation of reactive oxygen species metabolic process
aging
cell differentiation
protein heterooligomerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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