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Invitrogen
Gemin 4 Polyclonal Antibody
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Gemin 4 Antibody (PA5-88031) in WB
Western blot analysis of Gemin 4 in extracts of various cell lines. Samples were incubated with Gemin 4 Polyclonal antibody (Product # PA5-88031) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-88031
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human GEMIN4 (NP_0565362)
偶联物
形式
Liquid
浓度
2.29 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: LREISSAAAH SQPFAWKKKA LIIIWAKVLQ PHPVTPSDTE TRWQEDLFFS VGNMIPTINH TILFELLKSL EASGLFIQLL MALPTTICHA ELERFLEHVT VDTSAEDVAF FLDVWWEVMK HKGHPQDPLL SQFSAMAHKY LPALDEFPHP PKRLRSDPDA CPTMPLLAML LRGLTQIQSR ILGPGRKCCA LANLADMLTV FALTEDDPQE VSATVYLDKL ATVISVWNSD TQNPYHQQAL AEKVKEAERD VSLTSLAKLP SETIFVGCEF LHHLLREWGE ELQAVLRSSQ GTSYDSYRLC D; Positive Samples: HeLa, MCF-7, SH-SY5Y, Jurkat, Mouse brain, Mouse kidney, Mouse liver; Cellular Location: Cytoplasm, Nucleus, gem, nucleolus
靶标信息
The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies. The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Component of gems 4; DKFZp434B131; DKFZp434D174; Gem-associated protein 4; Gemin-4; HCC-associated protein 1; p97
基因别名: 4932415L08Rik; AU015359; GEMIN4; HC56; HCAP1; HHRF-1; p97
UniProt ID: (Human) P57678
Entrez Gene ID: (Human) 50628, (Mouse) 276919
