Rockland

Glutamate Dehydrogenase Polyclonal Antibody

Name: Glutamate Dehydrogenase Polyclonal Antibody
Brand: Rockland
SKU: 100-4158

查看其他13个GluD1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Glutamate Dehydrogenase Antibody in Western Blot (WB)

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Glutamate Dehydrogenase Antibody (100-4158) in WB

Western blot analysis is shown using Rocklands anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 µm nitrocellulose. The blot was incubated wi... View More

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Immunogen image

Immunogen Image Glutamate Dehydrogenase Polyclonal Antibody

产品信息

100-4158

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

酶联免疫吸附实验 (ELISA)

1:4,000-1:16,000

免疫沉淀 (IP)

1:1,000-1:3,000

产品规格

种属反应

Bovine

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

85 mg/mL

保存液

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

靶标信息

Glutamate dehydrogenase, GluD1, is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

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