Invitrogen

F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ 488

Name: F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor\u2122 488
Brand: Invitrogen
SKU: SA5-10382-AF488

高级验证

查看其他24个Goat anti-Mouse IgG2b抗体

说明书

问题与答案

技术支持

说明书

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

Mouse IgG2b Secondary Antibody in Immunocytochemistry (ICC/IF)

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Mouse IgG2b Secondary Antibody (SA5-10382-AF488) in ICC/IF

Immunofluorescence analysis of F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # SA5-10382-AF488) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer at room temperature. The cells were stained with Lamin A/C Monoclonal Antibody (mab636) (Product # MA3-1000, 1:200) diluted in SuperBlock™ Blocking Buffer (Product # 37515, 1:20) ov... View More

Mouse IgG2b Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

SA5-10382-AF488

应用

建议稀释比

免疫组化（石蜡） (IHC (P))

1:500

免疫细胞化学 (ICC/IF)

1:1,000-1:2500

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG, F(ab)

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Secondary Antibody

克隆号

27H27L7

抗原

Purified Mouse IgG

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

靶标

IgG2b

抗体形式

F(ab) Fragment

产品详细信息

F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ 488 is specific to Mouse IgG2b with no reactivity towards Mouse IgG1, Mouse IgG2a or Mouse IgG3. It shows no cross-reactivity to various species immunoglobulins tested.

This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency.

F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays.

Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

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