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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP
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Rabbit IgG (H+L) Secondary Antibody (T2191) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product # 702211, 2 µg/mL) and detected using Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP conjugate (Product # T2191) at dilutions 1:1,000 (Fig. 1) and 1:5,000 (Fig. 2). A 25 kDa band corresponding to PRDX6 wa... View More
产品信息
T2191
应用
建议稀释比
免疫印迹 (WB)
1:1,000-1:5,000
免疫组化 (IHC)
1:1,000
酶联免疫吸附实验 (ELISA)
1:1,000
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobin
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
TBS with 50% glycerol, 1.5% BSA
内含物
0.05% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
This Alkaline Phosphatase Conjugated secondary antibody can be used with the ELISA-Light Immunoassay, Western-Light, and Western-Star Western Blotting Detection Systems. This labeled secondary antibody ensures minimal nonspecific cross-reactivity. Life Technology has optimized its alkaline phosphatase secondary antibody conjugates for Western blotting or immunoassay procedures incorporating chemiluminescent 1,2-dioxetane enzyme substrates. Optimized for use in blotting applications with dioxetane-based substrate solutions (CSPD or CDP-Star substrates), providing consistent experiment-to-experiment results. High specific activity of conjugates provides high sensitivity of detection with dioxetane-based substrate solutions (CSPD or CDP-Star substrates). Low non-specific binding provides highest possible signal-to-noise in western blotting and immunoassay applications. Easy to Use The recommended initial working dilution for the labeled antibodies is 1:5,000 for AP conjugates. The conjugate is tested and optimized for low background with chemiluminescent detection procedures.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
