Invitrogen

H-Ras Recombinant Rabbit Monoclonal Antibody (4Q7M5)

Name: H-Ras Recombinant Rabbit Monoclonal Antibody (4Q7M5)
Brand: Invitrogen
SKU: MA5-35323

查看其他24个H-Ras抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

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H-Ras Antibody (MA5-35323) in WB

Western blot analysis of H-Ras in extracts of various cell lines. Samples were incubated with H-Ras Monoclonal antibody (Product # MA5-35323) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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产品信息

MA5-35323

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4Q7M5

抗原

Synthetic peptide

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2849225

靶标信息

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K-, N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibition of apoptosis. K-Ras mutations are common in pancreatic, colorectal and nonsmall-cell lung carcinomas; H-Ras mutations are common in bladder, kidney and thyroid carcinomas; N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinaoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; GTPase HRas; GTPase Hras1; H-ras 1 protein; H-Ras-1; Harvey ras1 protein; Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog; Harvey rat sarcoma virus oncogene 1; p21ras; Transforming protein p21

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基因别名: c-H-ras; c-Ha-ras; c-rasHa; H-ras; Ha-ras; Harvey-ras; Hras; Hras-1; HRAS1; Kras2; ras

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UniProt ID: (Mouse) Q61411, (Rat) P20171

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Entrez Gene ID: (Mouse) 15461, (Rat) 293621

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功能

nucleotide binding protein binding GTP binding protein C-terminus binding small GTPase

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