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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HA Tag Antibodies

          Invitrogen

          HA Tag Polyclonal Antibody

          高级验证
          19 篇参考文献
          查看其他62个HA Tag抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite HA Tag Polyclonal Antibody

          • 抗体检测数据 (5)
          • 高级验证 (1)
          HA Tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          HA Tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 6

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          HA Tag Antibody (PA1-985) in WB

          Western blot was performed using Anti-HA Tag Polyclonal Antibody (Product # PA1-985) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3, 40 µg (Lane 3), HA-H3, 20 µg (Lane 4), HA-H3, 10 µg (Lane 5), HA-H3, 5 µg (Lane 6), p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8 and 25 ng Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred ont... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          HA Tag Antibody in Western Blot (WB)
          HA Tag Antibody in Western Blot (WB)
          HA Tag Antibody in Western Blot (WB)
          HA Tag Antibody in Immunoprecipitation (IP)
          HA Tag Antibody in ChIP Assay (ChIP)
          HA Tag Antibody

          产品信息

          PA1-985

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:5,000
          查看7篇已发表文章 7篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看2篇已发表文章 2篇已发表文章

          流式细胞分析 (Flow)

          -
          查看2篇已发表文章 2篇已发表文章

          免疫沉淀 (IP)

          5 µg
          查看1篇已发表文章 1篇已发表文章

          染色质免疫沉淀 实验 (ChIP)

          2.5 µg/10^6 cells
          查看1篇已发表文章 1篇已发表文章

          亲和纯化 (AP)

          -
          查看3篇已发表文章 3篇已发表文章

          其他PubMed (Misc)

          -
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Tag

          已发表种属

          C. elegans, Tag

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic peptide corresponding to the residues Y P Y D V P D Y A of the HA epitope of influenza hemagglutinin.

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_559366

          产品详细信息

          PA1-985 detects HA tagged proteins. This antibody is specific for the HA tag and does not detect other proteins.

          PA1-985 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of HA-tagged proteins.

          The PA1-985 immunogen is a synthetic peptide corresponding to the residues Y P Y D V P D Y A of the HA epitope of influenza hemagglutinin.

          靶标信息

          HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          It has to be done as per old AB suggested Products section.
          guarantee_icon

          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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