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Invitrogen
HGF Recombinant Rabbit Monoclonal Antibody (7H 6L1)
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HGF Antibody (701283) in ICC/IF
Immunofluorescent analysis of HGF in HepG2 cells using a HGF recombinant rabbit monoclonal antibody (Product # 701283) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of HGF.
产品信息
701283
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
7H 6L1
抗原
Recombinant protein corresponding to amino acids 27-169 of human HGF
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin A; Hepatopoietin-A; HepatopoietinA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; Scatter factor; scatter factor); SF
基因别名: DFNB39; F-TCF; HGF; HGFB; HPTA; SF
UniProt ID: (Human) P14210
Entrez Gene ID: (Human) 3082
activation of MAPK activity
epithelial to mesenchymal transition
liver development
platelet degranulation
proteolysis
mitotic nuclear division
positive regulation of cell proliferation
negative regulation of autophagy
positive regulation of phosphatidylinositol 3-kinase signaling
hyaluronan metabolic process
positive regulation of cell migration
organ regeneration
positive regulation of myelination
negative regulation of interleukin-6 production
positive regulation of interleukin-10 production
negative regulation of peptidyl-serine phosphorylation
cellular response to hepatocyte growth factor stimulus
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of osteoblast differentiation
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
negative regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
myoblast proliferation
cell chemotaxis
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
positive regulation of neuron projection regeneration
negative regulation of release of cytochrome c from mitochondria
regulation of p38MAPK cascade
negative regulation of hydrogen peroxide-mediated programmed cell death
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of tau-protein kinase activity
positive regulation of DNA biosynthetic process
