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Invitrogen
HLA-ABCE Monoclonal Antibody (TP25.99SF)
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HLA-ABCE Antibody (MA5-44040) in WB
Western Blot analysis of HLA-ABCE using HLA-ABCE Monoclonal Antibody (TP25.99SF) (Product # MA5-44040). Analysis was performed on whole cell extracts (RIPA lysis buffer) of JAR, JEG3, Raji, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody TP25.99SF (2 µg/mL), followed by Goat-anti-Mouse IgG (green). Mouse IgG1 anti-GAPDH monoclonal antibody conjugated wit... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-44040
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
TP25.99SF
抗原
IFN-gamma-treated human melanoma cells Colo 38
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
Western blotting: non-reducing conditions.
靶标信息
HLA-A, -B, and -C are major histocompatibility complex (MHC)-class I antigens. Like other class I molecules (i.e. HLA-E, -F, -G), HLA-A, -B, and -C are heterodimers consisting of a 40 - 45 kDa transmembrane glycoproteic alpha-chain, noncovalently combined to the invariant B2-microglobulin.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA-A; HLA-B; HLA-C; HLA-Cw; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; MHC class I antigen E
基因别名: HLA-6.2; HLA-A; HLA-B; HLA-C; HLA-E; HLAA; HLAB; HLAC; HLAE
Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107, (Human) 3133
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
