NeoBiotechnologies

HLA-E (Major histocompatibility complex, class I, E) Monoclonal Antibody (HLAE/9467)

Name: HLA-E (Major histocompatibility complex, class I, E) Monoclonal Antibody (HLAE\/9467)
Brand: NeoBiotechnologies
SKU: 3133-MSM1-P1

查看其他26个HLA-E抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

HLA-E (Major histocompatibility complex, class I, E) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 3

HLA-E (Major histocompatibility complex, class I, E) Antibody (3133-MSM1-P1) in IHC (P)

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-E Mouse Monoclonal Antibody (HLAE/9467). Inset: PBS instead of primary antibody; secondary only negative control.

HLA-E (Major histocompatibility complex, class I, E) Antibody in Western Blot (WB)

HLA-E (Major histocompatibility complex, class I, E) Antibody in SDS-PAGE (SDS-PAGE)

Product Image Gallery

Immunogen image

Immunogen Image HLA-E (Major histocompatibility complex, class I, E) Monoclonal Antibody (HLAE/9467)

产品信息

3133-MSM1-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HLAE/9467

抗原

Recombinant fragment (around aa 1-200) of human HLAE protein (exact sequence is proprietary).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A

保存液

PBS with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Product cellular localization: Cell membrane.

Positive control: Liver: An at least moderate staining should be seen of sinusoids and Kupffer cells. Kidney: An at least moderate staining should be seen in glomerular capillaries.

Tissue specificity: Expressed in professional APCs: monocyte/macrophages, dendritic cells and B cells (at protein level) (PubMed:31495665, PubMed:23783831, PubMed:19830726). Expressed in thymic epithelial cells (at protein level) (PubMed:23783831).

靶标信息

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; lymphocyte antigen; MHC 1; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1

查看更多收起

基因别名: EA1.2; EA2.1; HLA-6.2; HLA-E; HLAE; MHC; QA1

查看更多收起

UniProt ID: (Human) P13747

查看更多收起

Entrez Gene ID: (Human) 3133

查看更多收起

功能

receptor binding beta-2-microglobulin binding MHC class I protein binding peptide antigen binding natural killer cell lectin-like receptor binding major histocompatibility complex protein

参与通路