Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Rockland
HSF1 Monoclonal Antibody (4B4), HRP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
200-503-W89
Product Specifications
Species Reactivity
Bovine,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4B4
Immunogen
Anti-HSF1 Antibody was produced by repeated immunization of rats with a purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1
Gene Aliases: AA960185; HSF; HSF1; Hsf1alpha; Hsf1beta; HSTF1
UniProt ID: (Bovine) Q08DJ8, (Human) Q00613, (Mouse) P38532
DNA binding
heat shock protein binding
chromatin DNA binding
identical protein binding
sequence-specific DNA binding
protein self-association
protein heterodimerization activity
Hsp90 protein binding
translation elongation factor binding
promoter-specific chromatin binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
DNA repair
mRNA processing
positive regulation of cell proliferation
negative regulation of protein complex assembly
cellular response to heat
cellular response to unfolded protein
cellular macromolecular complex assembly
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
mRNA transport
positive regulation of transcription from RNA polymerase II promoter in response to heat stress
cellular response to cadmium ion
cellular response to copper ion
cellular response to gamma radiation
cellular response to diamide
regulation of cellular response to heat
positive regulation of mRNA polyadenylation
cellular response to sodium arsenite
negative regulation of double-strand break repair via nonhomologous end joining
embryonic placenta development
protein phosphorylation
defense response
female meiotic division
spermatogenesis
negative regulation of cell proliferation
mRNA transcription
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
positive regulation of multicellular organism growth
embryonic process involved in female pregnancy
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
response to heat
transcription from RNA polymerase II promoter
regulation of spindle checkpoint
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support