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Invitrogen
Hexanoyl-Lysine adduct Monoclonal Antibody (5D9), PerCP
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Hexanoyl-Lysine adduct Antibody (MA5-45779) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with Hexanoyl-Lysine adduct monoclonal antibody (Product # MA5-45779) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-45779
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5D9
抗原
Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
偶联物
PerCP
PerCP
PerCP
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of MA5-45779 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
靶标信息
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker which is derived from the oxidation of omega-6 unsaturated fatty acid. HEL may be used as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HEL; HEL Adduct; Hexanoyl-Lys; Hexanoyl-Lys (HEL); Hexanoyl-Lys adduct
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