Leinco Technologies

I-A/I-E (MHC Class II) Monoclonal Antibody (M5/114.15.2), Functional Grade, In Vivo PLATINUM™

Name: I-A\/I-E (MHC Class II) Monoclonal Antibody (M5\/114.15.2), Functional Grade, In Vivo PLATINUM\u2122
Brand: Leinco Technologies
SKU: H270-100MG

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Immunogen image

Immunogen Image I-A/I-E (MHC Class II) Monoclonal Antibody (M5/114.15.2), Functional Grade, In Vivo PLATINUM™

产品信息

H270-100MG

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

流式细胞分析 (Flow)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

功能检测 (Functional)

Assay-dependent

抑制试剂 (Inhibition)

Assay-dependent

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

M5/114.15.2

抗原

C57BL/6 Spleen Cells

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偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

≥5.0 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.2-7.4

内含物

no preservative

保存条件

4°C short term, -80°C long term

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Specificity: Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.

Endotoxin Level: ≤ 0.5 EU/mg as determined by the LAL method.

Purity: ≥98% monomer by analytical SEC and >95% by SDS Page.

Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles.

靶标信息

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: A alpha; cell surface glycoprotein; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, beta 1; I-Ab; I-Ad; I-Aq; I-E beta MHC class II; I-Ed; I-Ek; IAalpha; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen A alpha isoform-004; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC H2-IE-beta cell surface glycoprotein; MHC-II I-A(d) alpha; response to metastatic cancers 1

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基因别名: Aalpha; Abeta; AI845868; H-2Aa; H-2Ab; H-2Eb; H2-Aa; H2-Ab; H2-Ab1; H2-iabeta; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1

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UniProt ID: (Mouse) P14434, (Mouse) P14483

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Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969

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功能

protein binding peptide antigen binding protein heterodimerization activity transcription factor binding toxic substance binding ubiquitin protein ligase binding repressing transcription factor binding major histocompatibility complex protein

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