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Invitrogen
IL-16 Polyclonal Antibody, PeproTech®
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IL-16 Antibody (500-P06-1MG) in WB
Western Blot: To detect Human IL-16 by Western Blot analysis IL-16 Polyclonal Antibody (Product # 500-P06-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
500-P06-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human IL-16 (129 a.a.)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.1-1.0 mg/mL
规格
1 mg
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: PDLNSSTDSA ASASAASDVS VESTAEATVC TVTLEKMSAG LGFSLEGGKG SLHGDKPLTI NRIFKGAASE QSETVQPGDE ILQLGGTAMQ GLTRFEAWNI IKALPDGPVT IVIRRKSLQS KETTAAGDS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-16. Anti-Human IL-16-specific antibody was purified by affinity chromatography employing an immobilized Human IL-16 matrix.
Sandwich ELISA: To detect Human IL-16 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-16 (500-P06Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-16.
Western Blot: To detect Human IL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
The protein encoded by IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of IL-16 undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ16806; FLJ42735; FLJ44234; H-IL-16; il 16; ILN; Interleukin; Interleukin16; M-IL-16; Pro-interleukin-16
基因别名: IL16
Entrez Gene ID: (Human) 3603
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