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Invitrogen
IL-2 Monoclonal Antibody (5H4)
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IL-2 Antibody (MM101) in ELISA
Sandwich ELISA analysis of mouse IL-2 was performed using the a Mouse IL-2 Colorimetric ELISA Kit (Product # EMIL2) by loading 50 µL per well of an anti-mouse IL-2 monoclonal antibody (Product # MM100) in quadruplicate at 0 (blue line), 50% (2.5 µg/mL, red line), and 100% (5 µg/mL, green line) displacement in a plate and then loading 50 µL per well of Mouse IL-2 Recombinant Protein (Product # SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/mL across a 5 µg/mL Mouse IL-2 (Product # MM101) pre-coated plate and incubating for 2 hours at 37°... View More
产品信息
MM101
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
5H4
抗原
Recombinant mouse IL-2
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MM101 targets IL-2 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM101 immunogen is recombinant mouse IL-2.
MM101 detects IL-2 which has a predicted molecular weight of approximately 17 kDa.
The MM101 mouse IL2 antibody (clone 5H4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM102B (biotinylated conjugate of clone 1A12). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody MM101 (clone 5H4) and biotinylated antibody MM102B (clone 1A12) have successfully been used in combination with recombinant mouse IL2 protein SMIL2 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
基因别名: Il-2; Il2
UniProt ID: (Mouse) P04351
Entrez Gene ID: (Mouse) 16183
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
immune system process
negative regulation of B cell apoptotic process
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
negative regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of immunoglobulin secretion
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
