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Invitrogen
IL5RA Polyclonal Antibody
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产品信息
PA1-24792
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified recombinant human IL-5 soluble receptor alpha, expressed in insect Sf 21 cells.
偶联物
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 5% trehalose
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: human blood-derived granulocytes.
The Neutralization Dose50 (ND50) for anti-human IL5RA is approximately 1-3 µg/mL in the presence of 0.5 ng/mL of recombinant human IL-5 using the human TF-1 cell line. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cell surface IL5RA mediated IL5 response on a responsive cell line, at a specific IL5 concentration. The exact concentration of antibody required to neutralize the human cell surface IL5RA mediated bioactivity is dependent on the IL5 concentration as well as on the number of IL5RA present on the cell surface (a function of the cell type and culture conditions).
靶标信息
Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD125; CD125 antigen; CDw125; IL-5 receptor subunit alpha; IL-5R subunit alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; Interleukin-5 receptor subunit alpha
基因别名: CD125; CDw125; HSIL5R3; IL5R; IL5RA
UniProt ID: (Human) Q14633
Entrez Gene ID: (Human) 3568
