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Invitrogen
ITGA5 Recombinant Rabbit Monoclonal Antibody (12H40L20)
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ITGA5 Antibody (702178) in WB
Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), U-937 (Lane 2), THP-1 (Lane 3) and HeLa (Lane 4). The blots were probed with Anti-Integrin alpha 5 beta 1 Recombinant Rabbit Monoclonal Antibody (Product # 702178, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:4000 dilution). A ~120 kDa band corresponding to Integrin alpha 5 beta 1 was observed across the cell lines tested... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
702178
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
12H40L20
抗原
Protein corresponding to human Integrin alpha5 beta1 (aa800-aa1049)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Horse, Cat, Bovine
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; Integrin alpha-5; Integrin alpha-F; VLA-5
基因别名: FNRA; ITGA5
Entrez Gene ID: (Human) 3678
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
