Huabio

Insulin B Chain Polyclonal Antibody

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Insulin B Chain Antibody (0807-11) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) a... View More

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1:10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1:10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1:200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunogen image

Insulin B Chain Polyclonal Antibody

Immunogen Image Insulin B Chain Polyclonal Antibody

产品信息

0807-11

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:10,000

-

酶联免疫吸附实验 (ELISA)

1:5,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human Insulin B Chain protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 0.2% BSA, 25% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Insulin; preproinsulin; proinsulin

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基因别名: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10

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UniProt ID: (Human) P01308

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Entrez Gene ID: (Human) 3630

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protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding growth factor

MAPK cascade negative regulation of acute inflammatory response glucose metabolic process regulation of transcription, DNA-templated regulation of cellular amino acid metabolic process ER to Golgi vesicle-mediated transport acute-phase response G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of gene expression positive regulation of phosphatidylinositol 3-kinase signaling glucose transport regulation of transmembrane transporter activity positive regulation of cell growth positive regulation of cell migration positive regulation of protein autophosphorylation activation of protein kinase B activity positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization negative regulation of NAD(P)H oxidase activity wound healing negative regulation of protein catabolic process glucose homeostasis positive regulation of MAPK cascade cellular protein metabolic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of gluconeogenesis positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of vasodilation positive regulation of vasodilation negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation positive regulation of lipid biosynthetic process regulation of protein secretion negative regulation of protein secretion positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipid catabolic process positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation positive regulation of protein localization to nucleus negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of feeding behavior