Huabio

Insulin B Chain Polyclonal Antibody

问题与答案

问题与答案

问题与答案

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抗体检测数据 (3)

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Insulin B Chain Antibody (0807-11) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) a... View More

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1:10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1:10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Insulin B Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1:200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1:200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

产品信息

0807-11

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:10,000

-

酶联免疫吸附实验 (ELISA)

1:5,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human Insulin B Chain protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 0.2% BSA, 25% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Insulin B refers to the B chain of insulin, one of the two peptide chains (A and B) produced after processing of proinsulin. It is a 30-amino acid peptide that plays a key role in insulin's biological activity, including receptor binding and regulation of glucose metabolism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: INSU1; INSU2; INSU3B; INSUA; INSUB; INSUC; Insulin; insulin splice variant; insulin variant; INS-splice; preproinsulin; proinsulin

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基因别名: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10; PNDM4

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UniProt ID: (Human) P01308

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Entrez Gene ID: (Human) 3630

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protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding receptor agonist activity

positive regulation of cytokine production negative regulation of acute inflammatory response glucose metabolic process regulation of transcription, DNA-templated acute-phase response G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of gene expression negative regulation of gene expression positive regulation of nitric oxide mediated signal transduction positive regulation of neuron projection development positive regulation of cell growth positive regulation of cell migration regulation of protein localization nitric oxide-cGMP-mediated signaling pathway TORC1 signaling wound healing negative regulation of protein catabolic process vasodilation glucose homeostasis positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of gluconeogenesis positive regulation of glycogen biosynthetic process positive regulation of translation negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation positive regulation of lipid biosynthetic process regulation of synaptic plasticity regulation of protein secretion negative regulation of protein secretion positive regulation of protein secretion cognition negative regulation of lipid catabolic process positive regulation of nitric-oxide synthase activity positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation positive regulation of protein localization to nucleus positive regulation of long-term synaptic potentiation cellular response to oxygen-containing compound negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of dendritic spine maintenance regulation of protein localization to plasma membrane negative regulation of reactive oxygen species biosynthetic process neuron projection maintenance negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of feeding behavior