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JunD Antibody (710701) in ICC/IF
Immunofluorescent analysis of JunD was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JunD Recombinant Rabbit Superclonal™ Antibody (Product # 710701) at 5 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 mi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
710701
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
2HCLC
抗原
Peptide, multiple (2-16, 132-149, 299-313)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with monkey, sheep, mouse, rat, zebra fish and bovine based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
JunD is a transcription factor which is coded by JunD gene. JunD transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) family of protein kinase. Jun proteins are capable of forming dimers with Fos, ATF and CREB family transcription factors to form the AP-1 complex that differentially regulates a variety of target genes involved in cellular growth, proliferation, differentiation and apoptosis. Also, JunD plays an important role in metabolism via modulation of IGF-1 signaling pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: activator protein 1; AP1; JUN D; jun D proto-oncogene; Oncogene Jun-D; transcription factor AP-1 subunit JunD; transcription factor jun-D; unnamed protein product
基因别名: AP-1
UniProt ID: (Human) P17535
Entrez Gene ID: (Human) 3727
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ligand-dependent nuclear receptor binding
enzyme binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
circadian rhythm
response to cold
response to water deprivation
response to light stimulus
response to mechanical stimulus
negative regulation of cardiac muscle hypertrophy
response to lipopolysaccharide
positive regulation of peroxisome proliferator activated receptor signaling pathway
regulation of cell proliferation
positive regulation of macrophage activation
response to peptide hormone
positive regulation of transcription from RNA polymerase II promoter
response to mercury ion
response to steroid hormone
epithelial cell proliferation
negative regulation of epithelial cell proliferation
regulation of cell cycle
cellular response to fatty acid
cellular response to hypoxia
response to forskolin
cellular response to polyamine macromolecule
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