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Invitrogen
KDEL Polyclonal Antibody, Alexa Fluor™ 555
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FIGURE: 1 / 4
KDEL Antibody (PA1-013-A555) in ICC/IF
Immunofluorescent analysis of KDEL/ER (orange) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a KDEL/ER Rabbit polyclonal antibody, AlexaFluor 555 conjugate (Product # PA1-013-A555), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,... View More
Product Details
PA1-013-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1013A555 detects KDEL from human, rat, mouse and hamster.
The PA1013A555 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; XAP-1 antigen
Gene Aliases: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; I79_019946; I79_020931; KDELR1; mBiP; MIF2; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1
RNA binding
calcium ion binding
protein binding
ATP binding
protein phosphatase binding
virion binding
low-density lipoprotein particle receptor binding
unfolded protein binding
glycoprotein binding
ATPase activity
enzyme binding
protein domain specific binding
ubiquitin protein ligase binding
ribosome binding
chaperone binding
misfolded protein binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
KDEL sequence binding
molecular_function
ER retention sequence binding
Hsp90 family chaperone
membrane trafficking regulatory protein
response to hypoxia
toll-like receptor signaling pathway
receptor-mediated endocytosis
protein transport
ER-associated ubiquitin-dependent protein catabolic process
retrograde protein transport, ER to cytosol
actin rod assembly
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
ATF6-mediated unfolded protein response
negative regulation of apoptotic process
regulation of phosphoprotein phosphatase activity
sequestering of calcium ion
cellular response to ATP
ER overload response
activation of signaling protein activity involved in unfolded protein response
cerebellum structural organization
cerebellar Purkinje cell layer development
substantia nigra development
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
endoplasmic reticulum unfolded protein response
positive regulation of protein ubiquitination
maintenance of protein localization in endoplasmic reticulum
IRE1-mediated unfolded protein response
PERK-mediated unfolded protein response
cellular response to glucose starvation
regulation of protein folding in endoplasmic reticulum
cellular response to antibiotic
cellular response to manganese ion
cellular response to interleukin-4
negative regulation of protein homodimerization activity
cell-cell adhesion
toxin transport
regulation of ATF6-mediated unfolded protein response
regulation of IRE1-mediated unfolded protein response
regulation of PERK-mediated unfolded protein response
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
protein folding
response to stress
proteolysis involved in cellular protein catabolic process
positive regulation of embryonic development
protein retention in ER lumen
intracellular protein transport
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
transport
biological_process
vesicle-mediated transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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