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Invitrogen
KDEL Monoclonal Antibody (1F2), FITC
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KDEL Antibody (MA5-45866) in WB
Western Blot analysis of Human Cervical cancer cells. Lane 2: Anti-KDEL (Product # MA5-45866). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with KDEL monoclonal antibody (Product # MA5-45866) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG:HRP at 1:5,000 for 1 hour at RT. Color Development: ECL solution fo... View More
产品信息
MA5-45866
产品规格
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1F2
抗原
KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
A 1:1,000 dilution of MA5-45866 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.
靶标信息
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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