Invitrogen

LRIG1 Polyclonal Antibody

Name: LRIG1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-47009

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1个已发表图片

2 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

LRIG1 Antibody in Immunocytochemistry (ICC/IF)

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LRIG1 Antibody (PA5-47009) in ICC/IF

Immunocytochemistry analysis of LRIG1 in immersion fixed bEnd.3 mouse endothelioma cell line. Samples were incubated in LRIG1 polyclonal antibody (Product # PA5-47009) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm.

LRIG1 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

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LRIG1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. bEnd.3 mouse endothelioma cells were transfected with LRIG1 siRNA and decrease in signal intensity was observed in Western blot application using LRIG1 Polyclonal Antibody (Product # PA5-27150). {KD}

Immunogen image

Immunogen Image LRIG1 Polyclonal Antibody

产品信息

PA5-47009

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

5-15 µg/mL

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流式细胞分析 (Flow)

0.25 µg per million cells

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse LRIG1 Ala37-Thr794

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2608300

产品详细信息

In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) LRIG1 is observed and less than 5% cross-reactivity with rhLRIG3 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

LRIG1 protein Q96JA1 is coded by a gene on a chromosome band 3p14.3, this region is known to be deleted in various human cancers. LRIG1 is considered to be a tumor suppressor gene in humans. LRIG1 is an integral cell-surface membrane protein that is expressed by specific cells in various human tissues and that its 143-kDa form might be cleaved into 111-kDa and 32-kDa fragments. The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。