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Agrisera
Linamarase Polyclonal Antibody
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Linamarase Antibody (AS15-2862) in WB
Samples: Latex - approximately 20 µL dissolved in 200 µL of extraction buffer Leaf (cassava) - 2 leaf discs (A=78.5 mm 2), snap frozen in liquid nitrogen and homogenized with pistil in 200 µL extraction buffer 10 µL of extract, is mixed with sample buffer (Bio Rad XT), reducing agent (Bio Rad XT) and Mili Q water to a final volume of 35 µL - 30 µLl of this extract is separated on 12% Bis-Tris (Bio-Rad Criterion) gel and run at 200 V for 1 h in XT MOPS buffer. Blotting is done to PVDF membrane with Bio-Rad Criterion, 100 V/30 min. Membranes... View More
产品信息
AS15-2862
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Manihot esculenta linamarase protein sequence.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Manihot esculenta
靶标信息
Linamarase is a cyanogenic beta-glucosidase encoded in cyanogenic plants (classically at the Li locus in white clover) and is localized predominantly outside the cytoplasm in the apoplast/cell wall, consistent with its role in initiating cyanogenesis only after tissue disruption brings enzyme and substrate into contact. It is synthesized as a secreted glycoprotein precursor containing an N-terminal signal peptide and multiple N-linked glycosylation sites, and the mature enzyme accumulates as an oligomeric protein composed of similar-sized subunits of roughly 60-63 kDa, with native species frequently observed as dimers/oligomers and, in cassava, multiple acidic isozymic forms. Functionally, linamarase catalyzes hydrolysis of the cyanogenic glucosides linamarin (and the related lotaustralin), releasing glucose and producing an unstable cyanohydrin that can decompose to hydrogen cyanide; this compartmentalized "enzyme in apoplast, substrate in cells" arrangement underpins a rapid, damage-activated chemical defense against herbivory and pathogens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: beta-D-glucosidase; beta-glucoside glucohydrolase; cyanogenic beta-glucosidase; Li
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