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Invitrogen
Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Biotin, eBioscience™
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Ly-6A/E (Sca-1) Antibody (13-5981-81) in WB
Nature cell biology 2015 -
Figure 5 Diet-induced adipocyte precursor activation and adipogenesis requires Akt2 in adipocyte lineage cells ( A ) Western blot for AKT1 and AKT2 in lysates from SVF or FACS-sorted APs from the SWAT (S) or VWAT (V) depot pooled from 12 wild-type animals. Uncropped blots are shown in Supplementary Figure 7A-B . ( B ) Western blot of lysates from APs enriched from SVF via Sca-1 bead pull down (see methods) ... View More
产品信息
13-5981-81
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Fish,
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
D7
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
4°C, do not freeze
运输条件
Wet ice
RRID
产品详细信息
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.
Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
