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Invitrogen
Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-eFluor™ 610, eBioscience™
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Ly-6A/E (Sca-1) Antibody (61-5981-80) in Flow
Staining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82), and 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse Ly-6A/E (Sca-1) PE-eFluor® 610 (right). Lineage negative/low viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
产品信息
61-5981-80
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Fish,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
D7
偶联物
激发/发射光谱
565/606 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.
Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
