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Invitrogen
MAP2 Monoclonal Antibody (AP18)
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MAP2 Antibody (MA5-12826) in ICC/IF
Immunofluorescence analysis of MAP2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MAP2 Monoclonal Antibody (AP18) (Product # MA5-12826) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More
产品信息
MA5-12826
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Guinea pig,
Human,
Mouse,
Rat,
Xenopus
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
AP18
抗原
Bovine brain microtubule associated protein 2
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-12826 has been successfully used in immunofluorescence analysis of Map2 in human glutamate neurons derived from iPSCs.
靶标信息
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
