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Invitrogen
MCAK Polyclonal Antibody
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FIGURE: 1 / 6
MCAK Antibody (PA5-79554) in ICC/IF
Immunocytochemistry analysis of MCAK using anti-MCAK antibody (Product # PA5-79554) . MCAK was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MCAK antibody (Product # PA5-79554) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-79554
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MCAK recombinant protein (Position: G531-Q725).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell. IHC: Mouse Testis Tissue, Rat Testis Tissue, Rat Thymus Tissue. ICC/IF: U20S cell. Flow: K562 cell.
Target Information
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: kinesin-like 6; Kinesin-like protein 6; Kinesin-like protein KIF2C; kinesin-related protein 2; MCAK; Mitotic centromere-associated kinesin; RP11-269F19.1; testis tissue sperm-binding protein Li 68n
Gene Aliases: 4930402F02Rik; CT139; ESTM5; KIF2C; KNSL6; KRP2; MCAK; X83316
UniProt ID: (Human) Q99661, (Mouse) Q922S8
Entrez Gene ID: (Human) 11004, (Mouse) 73804, (Rat) 171529
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based movement
microtubule depolymerization
sister chromatid cohesion
mitotic nuclear division
mitotic metaphase plate congression
cell proliferation
antigen processing and presentation of exogenous peptide antigen via MHC class II
establishment or maintenance of microtubule cytoskeleton polarity
cell division
regulation of chromosome segregation
cell cycle
chromosome segregation
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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